D4692
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 | Immunoblotting Nuclear extracts of HEK 293T cells were separated on SDS-PAGE, blotted with decreasing amounts of Rabbit Anti-DNMT1 (Cat. No. D4692) and developed with Goat anti-Rabbit IgG-Peroxidase conjugate (Cat. No. A0545) using a chemiluminescent substrate. Lane 1: Antibody 2.5 μg/mL Lane 2: Antibody 1 μg/mL Lane 3: Antibody 0.5 μg/mL Lane 4: Antibody 1 μg/mL + 10 μg/mL non-relevant peptide Lane 5: Antibody 1 μg/mL + 10 μg/mL DNMT1 immunizing peptide |
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D4692
Sigma
Anti-DNMT1 antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-DNA methyltransferase |
| MDL number: | MFCD06201933 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 72-78 of human DNMT1, conjugated to KLH via an N-terminal added cysteine residue. The sequence is conserved in mouse and is different from the rat sequence by one amino acid. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~180 kDa |
| species reactivity | mouse, human |
| application(s) | immunoblotting: 0.8-1.2 mg/mL using nuclear extracts of 293T cells |
| microarray: suitable | |
| Gene Information | human ... DNMT1(1786) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
